Bullough, Per, Professor

Professor Per Bullough

School of Biosciences

Harrison Chair in Structural Biology

p.bullough@sheffield.ac.uk
+44 114 222 4245

Firth Court

Full contact details

Professor Per Bullough
School of Biosciences
Firth Court
Western Bank
��«Ӱҵ
S10 2TN

Profile

Career history

2020 - present: Harrison Chair in Structural Biology, University of ��«Ӱҵ
2001 - present: Professor, Dept. of Molecular Biology and Biotechnology, University of ��«Ӱҵ
2002 - 2005: BBSRC Research Development Fellow
1995 - 2001: Lecturer, Dept. of Molecular Biology and Biotechnology, University of ��«Ӱҵ
1994 - 1995: Staff Scientist, MRC Laboratory of Molecular Biology, Cambridge
1990 - 1994: Research Associate, Department of Biochemistry and Molecular Biology, Harvard University, USA
1989 - 1990: Research Associate, Howard Hughes Medical Institute, Harvard University, USA
1988 - 1989: Staff Scientist, MRC Laboratory of Molecular Biology, Cambridge
1985 - 1988: PhD, MRC Laboratory of Molecular Biology and Darwin College, University of Cambridge

Research interests

Our laboratory focuses on two challenging areas of structural biology - membrane proteins and very large macromolecular complexes. Our main technique is high resolution cryoelectron microscopy, which we often use to complement other structural techniques such as X-ray crystallography.

Current projects fall under the following main headings:

Structure and assembly of large protein complexes at two dimensional interfaces- bacterial spore assembly, bacterial cell surfaces, photosynthetic proteins, bacterial toxins.
Energy transduction in membrane systems - photosynthesis, transporters.
Electron microscopy of macromolecular assemblies.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Stevenson SR, Tzokov SB, Lahiri I, Ayscough KR & Bullough PA (2025) . Structure.
Lanzoni-Mangutchi P, Banerji O, Wilson J, Barwinska-Sendra A, Kirk JA, Vaz F, O’Beirne S, Baslé A, El Omari K, Wagner A , Fairweather NF et al (2022) . Nature Communications, 13(1).
Janganan TK, Mullin N, Dafis-Sagarmendi A, Brunt J, Tzokov SB, Stringer S, Moir A, Chaudhuri RR, Fagan RP, Hobbs JK & Bullough PA (2020) . mSphere, 5(-), ---.
Pasquina-Lemonche L, Burns J, Turner RD, Kumar S, Tank R, Mullin N, Wilson JS, Chakrabarti B, Bullough PA, Foster SJ & Hobbs JK (2020) . Nature, 582(7811), 294-297.
Wilson JS, Churchill-Angus AM, Davies SP, Sedelnikova SE, Tzokov SB, Rafferty JB, Bullough PA, Bisson C & Baker PJ (2019) . Nature Communications, 10(1), ---.
Dix SR, Owen HJ, Sun R, Ahmad A, Shastri S, Spiewak HL, Mosby DJ, Harris MJ, Batters SL, Brooker TA , Tzokov SB et al (2018) . Nature Communications, 9(-), ---.
Terry C, Jiang S, Radford DS, Qiang W, Tzokov S, Moir A & Bullough PA (2017) . Molecular Microbiology, 104(4), 539-552.
Kailas L, Terry C, Abbott N, Taylor R, Mullin N, Tzokov SB, Todd SJ, Wallace BA, Hobbs JK, Moir A & Bullough PA (2011) . Proc Natl Acad Sci U S A, 108(38), 16014-16019.
Conroy MJ, Durand A, Lupo D, Li X-D, Bullough PA, Winkler FK & Merrick M (2007) . Proc Natl Acad Sci U S A, 104(4), 1213-1218.
Karrasch S, Bullough PA & Ghosh R (1995) . EMBO J, 14(4), 631-638.
Bullough PA, Hughson FM, Skehel JJ & Wiley DC (1994) . Nature, 371(6492), 37-43.

All publications

Journal articles

Stevenson SR, Tzokov SB, Lahiri I, Ayscough KR & Bullough PA (2025) . Structure.
Lanzoni-Mangutchi P, Banerji O, Wilson J, Barwinska-Sendra A, Kirk JA, Vaz F, O’Beirne S, Baslé A, El Omari K, Wagner A , Fairweather NF et al (2022) . Nature Communications, 13(1).
Salamaga B, Kong L, Pasquina-Lemonche L, Lafage L, von und zur Muhlen M, Gibson JF, Grybchuk D, Tooke AK, Panchal V, Culp EJ , Tatham E et al (2021) . Proceedings of the National Academy of Sciences, 118(44), e2106022118-e2106022118.
Janganan TK, Mullin N, Dafis-Sagarmendi A, Brunt J, Tzokov SB, Stringer S, Moir A, Chaudhuri RR, Fagan RP, Hobbs JK & Bullough PA (2020) . mSphere, 5(-), ---.
Pasquina-Lemonche L, Burns J, Turner RD, Kumar S, Tank R, Mullin N, Wilson JS, Chakrabarti B, Bullough PA, Foster SJ & Hobbs JK (2020) . Nature, 582(7811), 294-297.
Wilson JS, Churchill-Angus AM, Davies SP, Sedelnikova SE, Tzokov SB, Rafferty JB, Bullough PA, Bisson C & Baker PJ (2019) . Nature Communications, 10(1), ---.
Ji S, Dix SR, Aziz AA, Sedelnikova SE, Baker PJ, Rafferty JB, Bullough PA, Tzokov SB, Agirre J, Li F-L & Rice DW (2019) . Journal of Biological Chemistry, 294(48), 18077-18091.
Hill CJ, Fleming JR, Mousavinejad M, Nicholson R, Tzokov SB, Bullough PA, Bogomolovas J, Morgan MR, Mayans O & Murray P (2019) . Advanced Materials, 31(17), 1807521-1807521.
Dix SR, Owen HJ, Sun R, Ahmad A, Shastri S, Spiewak HL, Mosby DJ, Harris MJ, Batters SL, Brooker TA , Tzokov SB et al (2018) . Nature Communications, 9(-), ---.
Terry C, Jiang S, Radford DS, Qiang W, Tzokov S, Moir A & Bullough PA (2017) . Molecular Microbiology, 104(4), 539-552.
Janganan TK, Mullin N, Tzokov SB, Stringer S, Fagan RP, Hobbs JK, Moir A & Bullough PA (2016) . Food Microbiology, 59, 205-212.
Ma C, Hao Z, Huysmans G, Lesiuk A, Bullough P, Wang Y, Bartlam M, Phillips SE, Young JD, Goldman A , Baldwin SA et al (2015) . PLOS ONE, 10(11), e0143010-e0143010.
Jiang S, Wan Q, Krajcikova D, Tang J, Tzokov SB, Barak I & Bullough PA (2015) . Molecular Microbiology, 97(2), 347-359.
Adams NBP, Marklew CJ, Qian P, Brindley AA, Davison PA, Bullough PA & Hunter CN (2014) . Biochemical Journal, 464(3), 315-322.
Yamashita M, Shepherd M, Booth WI, Xie H, Postis V, Nyathi Y, Tzokov SB, Poole RK, Baldwin SA & Bullough PA (2014) . J Biol Chem, 289(33), 23177-23188.
Qian P, Papiz MZ, Jackson PJ, Brindley AA, Ng IW, Olsen JD, Dickman MJ, Bullough PA & Hunter CN (2013) . Biochemistry, 52(43), 7575-7585.
Huysmans GHM, Chan N, Baldwin JM, Postis VLG, Tzokov SB, Deacon SE, Yao SYM, Young JD, McPherson MJ, Bullough PA & Baldwin SA (2012) . Biochem J, 445(2), 157-166.
Qian P, Marklew CJ, Viney J, Davison PA, Brindley AA, Söderberg C, Al-Karadaghi S, Bullough PA, Grossmann JG & Hunter CN (2012) . J Biol Chem, 287(7), 4946-4956.
Glover CAP, Postis VLG, Charalambous K, Tzokov SB, Booth WI, Deacon SE, Wallace BA, Baldwin SA & Bullough PA (2011) . J Struct Biol, 176(3), 419-424.
Kailas L, Terry C, Abbott N, Taylor R, Mullin N, Tzokov SB, Todd SJ, Wallace BA, Hobbs JK, Moir A & Bullough PA (2011) . Proc Natl Acad Sci U S A, 108(38), 16014-16019.
Terry C, Shepherd A, Radford DS, Moir A & Bullough PA (2011) . PLoS One, 6(8), e23801.
Glover CAP, Postis VLG, Charalambous K, Tzokov SB, Booth WI, Deacon SE, Wallace BA, Baldwin SA & Bullough PA (2011) . Journal of Structural Biology, 176(3), 419-424.
Charalambous K, O'Reilly AO, Bullough PA & Wallace BA (2009) . Biochim Biophys Acta, 1788(6), 1279-1286.
Ball DA, Taylor R, Todd SJ, Redmond C, Couture-Tosi E, Sylvestre P, Moir A & Bullough PA (2008) . Mol Microbiol, 68(4), 947-958.
Baboolal TG, Conroy MJ, Gill K, Ridley H, Visudtiphole V, Bullough PA & Lakey JH (2008) . Structure, 16(3), 371-379.
Qian P, Bullough PA & Hunter CN (2008) . J Biol Chem, 283(20), 14002-14011.
Hunt S, Moir AJG, Tzokov S, Bullough PA, Artymiuk PJ & Green J (2008) . Microbiology, 154(Pt 2), 633-642.
Qian P, Bullough P & Hunter N (2007) Single particle analysis of the dimeric RC-LH1-PufX complex from Rhodobacter sphaeroides. PHOTOSYNTH RES, 91(2-3), 171-171.
Conroy MJ, Durand A, Lupo D, Li X-D, Bullough PA, Winkler FK & Merrick M (2007) . Proc Natl Acad Sci U S A, 104(4), 1213-1218.
Tzokov SB, Wyborn NR, Stillman TJ, Jamieson S, Czudnochowski N, Artymiuk PJ, Green J & Bullough PA (2006) . J Biol Chem, 281(32), 23042-23049.
Merrick M, Javelle A, Durand A, Severi E, Thornton J, Avent ND, Conroy MJ & Bullough PA (2006) . Transfus Clin Biol, 13(1-2), 97-102.
Conroy MJ, Bullough PA, Merrick M & Avent ND (2005) . Br J Haematol, 131(4), 543-551.
Stillman TJ, Upadhyay M, Norte VA, Sedelnikova SE, Carradus M, Tzokov S, Bullough PA, Shearman CA, Gasson MJ, Williams CH , Artymiuk PJ et al (2005) . Mol Microbiol, 57(4), 1101-1112.
Jelinska C, Conroy MJ, Craven CJ, Hounslow AM, Bullough PA, Waltho JP, Taylor GL & White MF (2005) . Structure, 13(7), 963-971.
Qian P, Hunter CN & Bullough PA (2005) . J Mol Biol, 349(5), 948-960.
Conroy MJ, Jamieson SJ, Blakey D, Kaufmann T, Engel A, Fotiadis D, Merrick M & Bullough PA (2004) . EMBO Rep, 5(12), 1153-1158.
Bahatyrova S, Frese RN, Siebert CA, Olsen JD, Van Der Werf KO, Van Grondelle R, Niederman RA, Bullough PA, Otto C & Hunter CN (2004) . Nature, 430(7003), 1058-1062.
Wyborn NR, Clark A, Roberts RE, Jamieson SJ, Tzokov S, Bullough PA, Stillman TJ, Artymiuk PJ, Galen JE, Zhao L , Levine MM et al (2004) . Microbiology, 150(Pt 5), 1495-1505.
Siebert CA, Qian P, Fotiadis D, Engel A, Hunter CN & Bullough PA (2004) . EMBO J, 23(4), 690-700.
Fotiadis D, Qian P, Philippsen A, Bullough PA, Engel A & Hunter CN (2004) . J Biol Chem, 279(3), 2063-2068.
Bullough PA (2004) . Microscopy and Microanalysis, 10(SUPPL. 2), 1496-1497.
Olsen JD, Robert B, Siebert CA, Bullough PA & Hunter CN (2003) . Biochemistry, 42(51), 15114-15123.
Qian P, Addlesee HA, Ruban AV, Wang P, Bullough PA & Hunter CN (2003) . J Biol Chem, 278(26), 23678-23685.
Reid JD, Siebert CA, Bullough PA & Hunter CN (2003) . Biochemistry, 42(22), 6912-6920.
Jamieson SJ, Wang P, Qian P, Kirkland JY, Conroy MJ, Hunter CN & Bullough PA (2002) . EMBO J, 21(15), 3927-3935.
Ruzheinikov SN, Burke J, Sedelnikova S, Baker PJ, Taylor R, Bullough PA, Muir NM, Gore MG & Rice DW (2001) . Structure, 9(9), 789-802.
Prince SM, Feron C, Janssens D, Lobet Y, Achtman M, Kusecek B, Bullough PA & Derrick JP (2001) . Acta Crystallogr D Biol Crystallogr, 57(Pt 8), 1164-1166.
Yin CC, Aldema-Ramos ML, Borges-Walmsley MI, Taylor RW, Walmsley AR, Levy SB & Bullough PA (2000) . Mol Microbiol, 38(3), 482-492.
Wallace AJ, Stillman TJ, Atkins A, Jamieson SJ, Bullough PA, Green J & Artymiuk PJ (2000) . Cell, 100(2), 265-276.
Collins R, Achtman M, Ford R, Bullough P & Derrick J (1999) Projection structure of reconstituted Opc outer membrane protein from Neisseria meningitidis. MOL MICROBIOL, 32(1), 217-219.
Bullough PA & Henderson R (1999) . J Mol Biol, 286(5), 1663-1671.
Subramaniam S, Lindahl M, Bullough PA, Faruqi AR, Tittor J, Oesterhelt D, Brown L, Lanyi J & Henderson R (1999) . Journal of Molecular Biology, 287, 145-161.
Walz T, Jamieson SJ, Bowers CM, Bullough PA & Hunter CN (1998) . J Mol Biol, 282(4), 833-845.
Henderson R, Bullough P, Lindahl M & Subramaniam S (1997) Structural changes underlying proton pumping by Bacteriorhodopsin. FASEB J, 11(9), A992-A992.
Karrasch S, Bullough PA & Ghosh R (1995) . EMBO J, 14(4), 631-638.
Skehel JJ, Bizebard T, Bullough PA, Hughson FM, Knossow M, Steinhauer DA, Wharton SA & Wiley DC (1995) . Cold Spring Harb Symp Quant Biol, 60, 573-580.
Bullough PA, Hughson FM, Treharne AC, Ruigrok RW, Skehel JJ & Wiley DC (1994) . J Mol Biol, 236(4), 1262-1265.
Bullough PA, Hughson FM, Skehel JJ & Wiley DC (1994) . Nature, 371(6492), 37-43.
Bullough PA & Tulloch PA (1991) . Ultramicroscopy, 35(2), 131-143.
Bullough PA & Tulloch PA (1990) . J Mol Biol, 215(1), 161-173.
Bullough PA & Henderson R (1990) . Biophys J, 58(3), 705-711.
Bullough PA (1990) . Electron Microsc Rev, 3(2), 249-267.
BULLOUGH P & HENDERSON R (1987) USE OF SPOT-SCAN PROCEDURE FOR RECORDING LOW-DOSE MICROGRAPHS OF BEAM-SENSITIVE SPECIMENS. ULTRAMICROSCOPY, 21(3), 223-229.
Al-Otaibi N, Baatsen P, Bhella D, Brescia R, Bullough P, Daum B, de Bruin R, Frank R, Kühlbrandt W, López-Iglesias C , McLaren M et al () . Faraday Discussions, 240, 142-151.
Bakker SE, Bhella D, Brescia R, Bullough P, Clare DK, Daum B, Frank RAW, Gold VAM, Jackson Hirst I, Kühlbrandt W , Lu P et al () . Faraday Discussions.
Al-Otaibi N, Aminian J. A, Anane RF, Baatsen P, Bakker SE, Bergeron J, Bharadwaj A, Bhella D, Braun T, Brescia R , Bullough P et al () . Faraday Discussions.
Bakker SE, Bergeron J, Bharadwaj A, Bhella D, Bullough P, Chau P-L, Frank RAW, Jakobi AJ, Joseph AP, Kühlbrandt W , Lahiri I et al () . Faraday Discussions.
Tank RKG, Lund VA, Kumar S, Turner RD, Lafage L, Pasquina Lemonche L, Bullough PA, Cadby A, Foster SJ & Hobbs JK () . ACS Nano.

Chapters

Shewring JR, Hodgson L, Bryant HL, Bullough PA, Weinstein JA & Verkade P (2021) , Methods in Cell Biology (pp. 69-87). Elsevier
Bullough P (2013) , Encyclopedia of Biophysics (pp. 581-586). Springer Berlin Heidelberg
Bullough PA, Qian P & Hunter CN (2009) , The Purple Phototrophic Bacteria (pp. 155-179). Springer Netherlands
Karrasch S, Bullough PA & Ghosh R (1995) , Photosynthesis: from Light to Biosphere (pp. 81-84). Springer Netherlands

Conference proceedings papers

Baboolal TG, Conroy MJ, Gill K, Bullough P & Lakey JH (2005) The colicin N- OmpF translocation complex. BIOPHYSICAL JOURNAL, Vol. 88(1) (pp 50A-51A)
Bullough PA, Jamieson SJ, Walz T, Hunter CN, Bowers CM, Karrasch S, Ghosh R, Henderson R & Subramaniam S (1999) Energy production in biological systems probed by electron crystallography. ELECTRON MICROSCOPY AND ANALYSIS 1999(161) (pp 127-132)
Bullough PA (1996) Conformational changes in influenza haemagglutinin at the pH of membrane fusion. OPTIONS FOR THE CONTROL OF INFLUENZA III, Vol. 1123 (pp 307-314)
Karrasch S, Bullough PA & Ghosh R (1995) 8.5 angstrom projection map of the light-harvesting complex I from Rhodospirillum rubrum reveals a ring composed of 16 subunits. PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE, VOL I (pp 81-84)
SKEHEL JJ, STEINHAUER D, WHARTON SA, BULLOUGH PA, HUGHSON FM, WATOWICH SJ & WILEY DC (1994) RECEPTOR BINDING AND MEMBRANE FUSION BY INFLUENZA HEMAGGLUTININ. CELLULAR RECEPTORS FOR ANIMAL VIRUSES (pp 187-193)

Datasets

Lemonche LP, Burns J, Turner R, Kumar S, Tank R, Mullin N, Wilson J, Chakrabarti B, Bullough P, Foster S & Hobbs J .

Preprints

Stevenson SR, Tzokov SB, Lahiri I, Ayscough KR & Bullough PA (2024) , Cold Spring Harbor Laboratory.
Wilson JS, Fortier L-C, Fagan RP & Bullough PA (2023) .
Banerji O, Lanzoni-Mangutchi P, Vaz F, Barwinska-Sendra A, Wilson J, Kirk J, O'Beirne S, Basle A, Omari KE, Wagner A , Fairweather N et al () .
Janganan TK, Mullin N, Dafis-Sagarmendi A, Brunt J, Tzokov SB, Stringer S, Moir A, Chaudhuri R, Fagan RP, Hobbs JK & Bullough PA () .
Mohasin M, Balbirnie-Cumming K, Fisk E, Prestwich EC, Russell CD, Marshall J, Pridans C, Allen SP, Shaw PJ, De Vos KJ , Hill CJ et al () , Cold Spring Harbor Laboratory.

Research group: I welcome applications from prospective home / EU / overseas PhD students and post-doctoral fellows.

Teaching activities

Level 3 Modules

MBB309 Membrane Protein Structure and Function (Module Coordinator)
MBB302 Physical Methods for Studying Biological Structures

Level 1 Modules

MBB162 Genetics