Waltho, Jon, Professor

Professor Jon Waltho

School of Biosciences

Gibson Chair in Biophysics

j.waltho@sheffield.ac.uk
+44 114 222 2717

Firth Court

Full contact details

Professor Jon Waltho
School of Biosciences
Firth Court
Western Bank
��«Ӱҵ
S10 2TN

Profile

Research focuses on the use of high field NMR spectroscopy to determine the structure and function of proteins and how they fold from their fully unfolded states.

Career history

2009: Awarded the Gibson Chair of Biophysics
2008: Professorial Appointment in Faculty of Life Sciences, University of Manchester
1999: Awarded a Personal Chair in Molecular Biology and Biotechnology
1997: Promoted to a Readership
1996: Awarded a Research Fellowship by the Lister Institute of Preventive Medicine
1996: Promoted to a Senior Lectureship
1990: Awarded a Krebs Institute Lectureship in the Department of Molecular Biology and Biotechnology, University of ��«Ӱҵ
1987 - 90: Postdoctoral research assistant, with Dr P. E. Wright, Department of Molecular Biology, The Scripps Research Institute, La Jolla, California
1986 - 87: Postdoctoral research assistant, with Dr J. G. Vinter, SmithKline French Research
1983 - 86: Postgraduate studentship, SERC funded, with Dr D. H. Williams in the University Chemical Laboratory and Emmanual College, Cambridge

Research interests

Our laboratory focuses on the use of high field NMR spectroscopy to determine the structure and function of proteins and how they fold from their fully unfolded states. Our studies address both fundamental aspects of protein biophysics and dynamics, and the investigation of the biomedical targets and their inhibition.

Recent highlights include the structure determination and characterisation of the solution dynamics of the human intracellular cysteine proteinase inhibitor stefin A. Proteins of this family inhibit enzymes central to the invasion of the body by foreign organisms (e.g. trypanosomes that cause African Sleeping sickness) and the entry of metastatic cancer cells into new tissues.

In addition, the absence of the protein stefin B was recently the first identified genetic cause of epilepsy.

Structure and dynamic measurements of stefins provide insights into means of developing small molecule pharmaceuticals that mimic the protective function of this class of proteins.

Understanding how proteins fold is both a major goal from a viewpoint of fundamental biochemistry, and is of growing biomedical importance owing its implication in a variety of neurodegenerative diseases.

Diseases ranging from Alzheimer’s, through amyloid angiopathy to the prion diseases, CJD and BSE, appear to utilise partially and misfolded states of proteins.

We have shown how NMR can be used to determine structural and dynamic information of such states, which provide a basis for identifying where and how to interrupt amyloidogenesis before the onset of neurodegeneration. We focus on three proteins in this regard, cystatin C, human prion protein and phosphoglycerate kinase (PGK).

Enzymes that catalyse phosphoryl transfer include kinases, ATPases and phosphatases, and therefore constitute arguably the most important group of enzymes. They increase the reaction rate by up to 10²⁰-fold - a truly remarkable rate increase.

Studies by our group, reported in , use a combination of X-ray crystallography, NMR, and computational analysis, using metal fluorides as analogues of the phosphate group.

They show how these enzymes use a combination of precise geometrical positioning, charge balance, and control of hydrogen bonds, to achieve their rate enhancements. They also show how much we still have to learn.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Griffin JL, Bowler MW, Baxter NJ, Leigh KN, Dannatt HRW, Hounslow AM, Blackburn GM, Webster CE, Cliff MJ & Waltho JP (2012) . Proc Natl Acad Sci U S A, 109(18), 6910-6915.
Xiaoxia L, Marston JP, Baxter NJ, Hounslow AM, Yufen Z, Blackburn GM, Cliff MJ & Waltho JP (2011) . J Am Chem Soc, 133(11), 3989-3994.
Cliff MJ, Bowler MW, Varga A, Marston JP, Szabó J, Hounslow AM, Baxter NJ, Blackburn GM, Vas M & Waltho JP (2010) . J Am Chem Soc, 132(18), 6507-6516.
Baxter NJ, Bowler MW, Alizadeh T, Cliff MJ, Hounslow AM, Wu B, Berkowitz DB, Williams NH, Blackburn GM & Waltho JP (2010) . Proc Natl Acad Sci U S A, 107(10), 4555-4560.
Baxter NJ, Hounslow AM, Bowler MW, Williams NH, Blackburn GM & Waltho JP (2009) . J Am Chem Soc, 131(45), 16334-16335.
Baxter NJ, Blackburn GM, Marston JP, Hounslow AM, Cliff MJ, Bermel W, Williams NH, Hollfelder F, Wemmer DE & Waltho JP (2008) . J Am Chem Soc, 130(12), 3952-3958.

All publications

Journal articles

Pellegrini E, Juyoux P, von Velsen J, Baxter NJ, Dannatt HRW, Jin Y, Cliff MJ, Waltho JP & Bowler MW (2024) . Structure, S0969-2126(24)00270.
Hosszu LLP, Sangar D, Batchelor M, Risse E, Hounslow AM, Collinge J, Waltho JP & Bieschke J (2023) . Journal of Molecular Biology, 435(15), 168158-168158.
Robertson AJ, Wilson AL, Burn MJ, Cliff MJ, Popelier PLA & Waltho JP (2021) . ACS Catalysis, 2021(11), 12840-12849.
Milanesi L, Trevitt CR, Whitehead B, Hounslow AM, Tomas S, Hosszu LLP, Hunter CA & Waltho JP (2021) . Magnetic Resonance, 2(2), 629-642.
Wood HP, Baxter NJ, Cruz-Navarrete FA, Trevitt CR, Hounslow AM & Waltho JP (2021) . Green Chemistry, 23(2), 752-762.
Hosszu LLP, Conners R, Sangar D, Batchelor M, Sawyer EB, Fisher S, Cliff MJ, Hounslow AM, McAuley K, Brady RL , Jackson GS et al (2020) . Communications Biology, 3(1).
Wood HP, Cruz-Navarrete FA, Baxter NJ, Trevitt CR, Robertson AJ, Dix SR, Hounslow AM, Cliff MJ & Waltho JP (2020) . Nature Communications, 11(1).
Wilson AL, Outeiral C, Dowd SE, Doig AJ, Popelier PLA, Waltho JP & Almond A (2020) . Communications Chemistry, 3(1).
Panova S, Cliff MJ, Macek P, Blackledge M, Jensen MR, Nissink JWM, Embrey KJ, Davies R & Waltho JP (2019) . Structure.
Xanthis I, Souilhol C, Serbanovic-Canic J, Roddie H, Kalli AC, Fragiadaki M, Wong R, Shah DR, Askari JA, Canham L , Akhtar N et al (2019) . Journal of Cell Science.
Czarnota S, Johannissen LO, Baxter NJ, Rummel F, Wilson AL, Cliff MJ, Levy CW, Scrutton NS, Waltho JP & Hay S (2019) . ACS Catalysis, 9(5), 4394-4401.
Cruz-Navarrete FA, Baxter NJ, Wood HP, Hounslow AM & Waltho JP (2019) . Biomolecular NMR Assignments, 13(2), 349-356.
Iorgu AI, Baxter NJ, Cliff MJ, Levy C, Waltho JP, Hay S & Scrutton NS (2018) . ACS Catalysis, 8(12), 11589-11599.
Johnson LA, Robertson AJ, Baxter NJ, Trevitt CR, Bisson C, Jin Y, Wood HP, Hounslow AM, Cliff MJ, Blackburn GM , Bowler MW et al (2018) . ACS Catalysis, 8(9), 8140-8153.
Bennet I, Finger LD, Baxter NJ, Ambrose B, Hounslow A, Thompson MJ, Exell JC, Shahari NNBM, Craggs TD, Waltho JP & Grasby JA (2018) . Nucleic Acids Research, 46(11), 5618-5633.
Macek P, Cliff MJ, Embrey KJ, Holdgate GA, Nissink JWM, Panova S, Waltho JP & Davies RA (2018) . Journal of Biological Chemistry, 293(24), 9301-9310.
Iorgu AI, Baxter NJ, Cliff MJ, Waltho JP, Hay S & Scrutton NS (2018) . Biomolecular NMR Assignments, 12(1), 79-83.
Serimbetov Z, Baxter NJ, Cliff MJ & Waltho JP (2017) . Biomolecular NMR Assignments, 11(2), 251-256.
Jin Y, Molt RW, Pellegrini E, Cliff MJ, Bowler MW, Richards NGJ, Blackburn GM & Waltho JP (2017) . Angewandte Chemie, 129(33), 9864-9867.
Jin Y, Molt Jr. RW, Pellegrini E, Cliff MJ, Bowler MW, Richards NGJ, Blackburn GM & Waltho JP (2017) . Angewandte Chemie International Edition, 56(33), 9732-9735.
Blackburn GM, Cherfils J, Moss GP, Richards NGJ, Waltho JP, Williams NH & Wittinghofer A (2017) . Pure and Applied Chemistry, 89(5), 653-675.
Jin Y, Richards NG, Waltho JP & Blackburn GM (2017) . Angewandte Chemie, 129(15), 4172-4192.
Blackburn GM, Jin Y, Richards NG & Waltho JP (2017) . Angewandte Chemie International Edition, 56(15), 4110-4128.
Czarnota S, Baxter NJ, Cliff MJ, Waltho JP, Scrutton NS & Hay S (2016) . Biomolecular NMR Assignments.
Jin Y, Molt RW, Waltho JP, Richards NGJ & Blackburn GM (2016) . Angewandte Chemie, 128(10), 3379-3383.
Jin Y, Molt RW, Waltho JP, Richards NGJ & Blackburn GM (2016) . Angewandte Chemie International Edition, 55(10), 3318-3322.
Briggs DC, Birchenough HL, Ali T, Rugg MS, Waltho JP, Ievoli E, Jowitt TA, Enghild JJ, Richter RP, Salustri A , Milner CM et al (2015) . Journal of Biological Chemistry, 290(48), 28708-28723.
Davis PJ, Holmes D, Waltho JP & Staniforth RA (2015) . Journal of Molecular Biology, 427(15), 2418-2434.
Kiraly P, Adams RW, Paudel L, Foroozandeh M, Aguilar JA, Timári I, Cliff MJ, Nilsson M, Sándor P, Batta G , Waltho JP et al (2015) . Journal of Biomolecular NMR, 62(1), 43-52.
Trevitt CR, Hosszu LLP, Batchelor M, Panico S, Terry C, Nicoll AJ, Risse E, Taylor WA, Sandberg MK, Al-Doujaily H , Linehan JM et al (2014) . Journal of Biological Chemistry, 289(37), 25497-25508.
Jin Y, Bhattasali D, Pellegrini E, Forget SM, Baxter NJ, Cliff MJ, Bowler MW, Jakeman DL, Blackburn GM & Waltho JP (2014) . Proceedings of the National Academy of Sciences, 111(34), 12384-12389.
Tsubery H, Krishnan R, Proschitsky M, Asp E, Lulu M, Gilead S, Gartner M, Gannon KS, Levenson JM, Schroeter S , Cullen V et al (2014) . Alzheimer's & Dementia, 10, P494-P494.
Krishnan R, Tsubery H, Proschitsky MY, Asp E, Lulu M, Gilead S, Gartner M, Wright J, Fisher RA, Waltho JP , Davis PJ et al (2014) . Journal of Molecular Biology.
Paudel L, Adams RW, Király P, Aguilar JA, Foroozandeh M, Cliff MJ, Nilsson M, Sándor P, Waltho JP & Morris GA (2013) . Angewandte Chemie, 125(44), 11830-11833.
Paudel L, Adams RW, Király P, Aguilar JA, Foroozandeh M, Cliff MJ, Nilsson M, Sándor P, Waltho JP & Morris GA (2013) . Angew Chem Int Ed Engl, 52(44), 11616-11619.
Krishnan R, Tsubery H, Waltho J, Proschitsky M, Lulu M, Asp E, Gilead S, Davies P, Gannon K, Masliah E , Levenson J et al (2013) . Alzheimer's & Dementia, 9, P361-P362.
Pudney CR, Guerriero A, Baxter NJ, Johannissen LO, Waltho JP, Hay S & Scrutton NS (2013) . J Am Chem Soc, 135(7), 2512-2517.
Jin Y, Cliff MJ, Baxter NJ, Dannatt HRW, Hounslow AM, Bowler MW, Blackburn GM & Waltho JP (2012) . Angewandte Chemie, 124(49), 12408-12411.
Milanesi L, Waltho JP, Hunter CA, Shaw DJ, Beddard GS, Reid GD, Dev S & Volk M (2012) . Proc Natl Acad Sci U S A, 109(48), 19563-19568.
Jin Y, Cliff MJ, Baxter NJ, Dannatt HRW, Hounslow AM, Bowler MW, Blackburn GM & Waltho JP (2012) . Angew Chem Int Ed Engl, 51(49), 12242-12245.
Paramore R, Morgan GJ, Davis PJ, Sharma CA, Hounslow A, Taler-Verčič A, Žerovnik E, Waltho JP, Cliff MJ & Staniforth RA (2012) . Frontiers in Molecular Neuroscience(AUG 2012), 1-30.
Blackburn GM, Bowler MW, Jin Y & Waltho JP (2012) . BIOCHEMISTRY-MOSCOW, 77(10), 1083-1096.
Kubota T, Hamazoe Y, Hashiguchi S, Ishibashi D, Akasaka K, Nishida N, Katamine S, Sakaguchi S, Kuroki R, Nakashima T & Sugimura K (2012) . Journal of Biological Chemistry, 287(17), 14023-14039.
Griffin JL, Bowler MW, Baxter NJ, Leigh KN, Dannatt HRW, Hounslow AM, Blackburn GM, Webster CE, Cliff MJ & Waltho JP (2012) . Proc Natl Acad Sci U S A, 109(18), 6910-6915.
Rauter AP, Dias C, Martins A, Branco I, Neng NR, Nogueira JM, Goulart M, Silva FVM, Justino J, Trevitt C & Waltho JP (2012) Non-toxic Salvia sclareoides Brot. extracts as a source of functional food ingredients: Phenolic profile, antioxidant activity and prion binding properties. Food Chemistry.
Rauter AP, Dias C, Martins A, Branco I, Neng NR, Nogueira JM, Goulart M, Silva FVM, Justino J, Trevitt C & Waltho JP (2012) . Food Chemistry, 132(4), 1930-1935.
Milanesi L, Hunter CA, Tzokova N, Waltho JP & Tomas S (2011) . Chemistry, 17(35), 9753-9761.
Jelinska C, Davis PJ, Kenig M, Zerovnik E, Kokalj SJ, Gunčar G, Turk D, Turk V, Clarke DT, Waltho JP & Staniforth RA (2011) . Biophys J, 100(9), 2268-2274.
Xiaoxia L, Marston JP, Baxter NJ, Hounslow AM, Yufen Z, Blackburn GM, Cliff MJ & Waltho JP (2011) . J Am Chem Soc, 133(11), 3989-3994.
Nicoll AJ, Trevitt CR, Tattum MH, Risse E, Quarterman E, Ibarra AA, Wright C, Jackson GS, Sessions RB, Farrow M , Waltho JP et al (2010) . Proc Natl Acad Sci U S A, 107(41), 17610-17615.
Hosszu LLP, Tattum MH, Jones S, Trevitt CR, Wells MA, Waltho JP, Collinge J, Jackson GS & Clarke AR (2010) . Biochemistry, 49(40), 8729-8738.
Nicoll AJ, Trevitt CR, Tattum MH, Risse E, Quaterman E, Ibarra AA, Jackson GS, Sessions RB, Farrow M, Waltho JP , Clarke AR et al (2010) A Pharmacological Chaperone for the Structured Domain of Human Prion Protein. PRION, 4(3), 130-130.
Cliff MJ, Bowler MW, Varga A, Marston JP, Szabó J, Hounslow AM, Baxter NJ, Blackburn GM, Vas M & Waltho JP (2010) . J Am Chem Soc, 132(18), 6507-6516.
Baxter NJ, Bowler MW, Alizadeh T, Cliff MJ, Hounslow AM, Wu B, Berkowitz DB, Williams NH, Blackburn GM & Waltho JP (2010) . Proc Natl Acad Sci U S A, 107(10), 4555-4560.
Marston JP, Cliff MJ, Reed MAC, Blackburn GM, Hounslow AM, Craven CJ & Waltho JP (2010) . J Mol Biol, 396(2), 345-360.
Bowler MW, Cliff MJ, Waltho JP & Blackburn GM (2010) . NEW J CHEM, 34(5), 784-794.
Baxter NJ, Hounslow AM, Bowler MW, Williams NH, Blackburn GM & Waltho JP (2009) . J Am Chem Soc, 131(45), 16334-16335.
Hosszu LLP, Trevitt CR, Jones S, Batchelor M, Scott DJ, Jackson GS, Collinge J, Waltho JP & Clarke AR (2009) . J Biol Chem, 284(33), 21981-21990.
Menon BRK, Waltho JP, Scrutton NS & Heyes DJ (2009) . J Biol Chem, 284(27), 18160-18166.
Hart T, Hosszu LLP, Trevitt CR, Jackson GS, Waltho JP, Collinge J & Clarke AR (2009) . Proc Natl Acad Sci U S A, 106(14), 5651-5656.
Golicnik M, Olguin LF, Feng G, Baxter NJ, Waltho JP, Williams NH & Hollfelder F (2009) . J Am Chem Soc, 131(4), 1575-1588.
Skerget K, Vilfan A, Pompe-Novak M, Turk V, Waltho JP, Turk D & Zerovnik E (2009) . Proteins, 74(2), 425-436.
Cliff MJ, Craven CJ, Marston JP, Hounslow AM, Clarke AR & Waltho JP (2009) . J Mol Biol, 385(1), 266-277.
Milanesi L, Jelinska C, Hunter CA, Hounslow AM, Staniforth RA & Waltho JP (2008) . Biochemistry, 47(51), 13620-13634.
Milanesi L, Tomas S, Hunter CA, Weinstein JA, Edge R, Navaratnam S, Waltho JP & Best J (2008) . Free Radic Biol Med, 45(9), 1271-1278.
Baxter NJ, Blackburn GM, Marston JP, Hounslow AM, Cliff MJ, Bermel W, Williams NH, Hollfelder F, Wemmer DE & Waltho JP (2008) . J Am Chem Soc, 130(12), 3952-3958.
Morgan GJ, Giannini S, Hounslow AM, Craven CJ, Zerovnik E, Turk V, Waltho JP & Staniforth RA (2008) . J Mol Biol, 375(2), 487-498.
Jenko Kokalj S, Guncar G, Stern I, Morgan G, Rabzelj S, Kenig M, Staniforth RA, Waltho JP, Zerovnik E & Turk D (2007) . J Mol Biol, 366(5), 1569-1579.
Wells MA, Jelinska C, Hosszu LLP, Craven CJ, Clarke AR, Collinge J, Waltho JP & Jackson GS (2006) . Biochem J, 400(3), 501-510.
Cliff MJ, Alizadeh T, Jelinska C, Craven CJ, Staniforth RA & Waltho JP (2006) . J Mol Biol, 364(4), 810-823.
Wells MA, Jackson GS, Jones S, Hosszu LLP, Craven CJ, Clarke AR, Collinge J & Waltho JP (2006) . Biochem J, 399(3), 435-444.
Baxter NJ, Olguin LF, Golicnik M, Feng G, Hounslow AM, Bermel W, Blackburn GM, Hollfelder F, Waltho JP & Williams NH (2006) . Proc Natl Acad Sci U S A, 103(40), 14732-14737.
Berrisford JM, Hounslow AM, Akerboom J, Hagen WR, Brouns SJJ, van der Oost J, Murray IA, Michael Blackburn G, Waltho JP, Rice DW & Baker PJ (2006) . J Mol Biol, 358(5), 1353-1366.
Reed MAC, Jelinska C, Syson K, Cliff MJ, Splevins A, Alizadeh T, Hounslow AM, Staniforth RA, Clarke AR, Craven CJ & Waltho JP (2006) . J Mol Biol, 357(2), 365-372.
Kenig M, Jenko-Kokalj S, Tusek-Znidaric M, Pompe-Novak M, Guncar G, Turk D, Waltho JP, Staniforth RA, Avbelj F & Zerovnik E (2006) . Proteins, 62(4), 918-927.
Baxter NJ, Roetzer A, Liebig H-D, Sedelnikova SE, Hounslow AM, Skern T & Waltho JP (2006) . J Virol, 80(3), 1451-1462.
Milanesi L, Hunter CA, Sedelnikova SE & Waltho JP (2006) . Chemistry, 12(4), 1081-1087.
Hosszu LLP, Wells MA, Jackson GS, Jones S, Batchelor M, Clarke AR, Craven CJ, Waltho JP & Collinge J (2005) . Biochemistry, 44(50), 16649-16657.
Jelinska C, Conroy MJ, Craven CJ, Hounslow AM, Bullough PA, Waltho JP, Taylor GL & White MF (2005) . Structure, 13(7), 963-971.
Syson K, Thirlway J, Hounslow AM, Soultanas P & Waltho JP (2005) . Structure, 13(4), 609-616.
Zhong Q, Watson MJ, Lazar CS, Hounslow AM, Waltho JP & Gill GN (2005) . Mol Biol Cell, 16(4), 2049-2057.
Trevitt CR, Craven CJ, Milanesi L, Syson K, Mattinen M-L, Perkins J, Annila A, Hunter CA & Waltho JP (2005) . Chem Biol, 12(1), 89-97.
Hosszu LLP, Jackson GS, Trevitt CR, Jones S, Batchelor M, Bhelt D, Prodromidou K, Clarke AR, Waltho JP & Collinge J (2004) . J Biol Chem, 279(27), 28515-28521.
Milanesi L, Reid GD, Beddard GS, Hunter CA & Waltho JP (2004) . Chemistry, 10(7), 1705-1710.
Japelj B, Waltho JP & Jerala R (2004) . Proteins, 54(3), 500-512.
Cliff MJ, Higgins LD, Sessions RB, Waltho JP & Clarke AR (2004) . J Mol Biol, 336(2), 497-508.
Sanders A, Jeremy Craven C, Higgins LD, Giannini S, Conroy MJ, Hounslow AM, Waltho JP & Staniforth RA (2004) . J Mol Biol, 336(1), 165-178.
Reed MAC, Hounslow AM, Sze KH, Barsukov IG, Hosszu LLP, Clarke AR, Craven CJ & Waltho JP (2003) . J Mol Biol, 330(5), 1189-1201.
Bailey S, Sedelnikova SE, Mesa P, Ayora S, Waltho JP, Ashcroft AE, Baron AJ, Alonso JC & Rafferty JB (2003) . J Biol Chem, 278(17), 15304-15312.
Mattinen M-L, Pääkkönen K, Ikonen T, Craven J, Drakenberg T, Serimaa R, Waltho J & Annila A (2002) . Biophys J, 83(2), 1177-1183.
Zerovnik E, Turk V & Waltho JP (2002) . Biochem Soc Trans, 30(4), 543-547.
Staniforth R, Giannini S, Higgins L & Waltho J (2002) Three-dimensional domain swapping in the folded and molten-globule states of cystatins, an amyloid-forming structural superfamily.. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 223, C36-C36.
Staniforth RA, Giannini S, Higgins LD, Conroy MJ, Hounslow AM, Jerala R, Craven CJ & Waltho JP (2001) . EMBO J, 20(17), 4774-4781.
Thaw P, Baxter NJ, Hounslow AM, Price C, Waltho JP & Craven CJ (2001) . Nat Struct Biol, 8(8), 701-704.
Jackson GS, Murray I, Hosszu LL, Gibbs N, Waltho JP, Clarke AR & Collinge J (2001) . Proc Natl Acad Sci U S A, 98(15), 8531-8535.
Trevitt CR, Batchelor M, Jackson GS, Clarke AR, Waltho JP, Holland S, Valler M, Snowdon M & Collinge J (2001) A rational approach to identifying treatments for prion diseases. FUNDAMENTAL & CLINICAL PHARMACOLOGY, 15, 112-112.
Craven CJ, Baxter NJ, Murray EH, Hill NJ, Martin JR, Ylinenjärvi K, Björk I, Waltho JP & Murray IA (2000) . Biochemistry, 39(51), 15783-15790.
Kahn SD, Booth PM, Waltho JP & Williams DH (2000) . J Org Chem, 65(24), 8406.
Staniforth RA, Dean JL, Zhong Q, Zerovnik E, Clarke AR & Waltho JP (2000) . Proc Natl Acad Sci U S A, 97(11), 5790-5795.
Collinge J, Collinge J, Jackson GS, Hosszu LLP, Power A, Hill AF, Kenney J, Saibil H, Craven CJ, Waltho JP & Clarke AR (2000) . Neurobiology of Aging, 21, 209-209.
Kahn SD, Booth PM, Waltho JP & Williams DH (2000) Erratum: Computer-assisted structure determination. Structure of the peptide moroidin from Laportea moroides (Journal of Organic Chemistry (1989) 54 (1901)). Journal of Organic Chemistry, 65(24), 8406-8406.
Baxter NJ, Thaw P, Higgins LD, Sedelnikova SE, Bramley AL, Price C, Waltho JP & Craven CJ (2000) Letter to the Editor: Backbone NMR assignment of the 19 kDa translationally controlled tumor-associated protein p23(fyp) from Schizosaccharomyces pombe. J BIOMOL NMR, 16(1), 83-84.
Baxter NJ, Thaw P, Higgins LD, Sedelnikova SE, Bramley AL, Price C, Waltho JP & Craven CJ (2000) . J Biomol NMR, 16(1), 83-84.
Hosszu LL, Baxter NJ, Jackson GS, Power A, Clarke AR, Waltho JP, Craven CJ & Collinge J (1999) . Nat Struct Biol, 6(8), 740-743.
Zerovnik E, Virden R, Jerala R, Kroon-Zitko L, Turk V & Waltho JP (1999) Differences in the effects of TFE on the folding pathways of human stefins A and B.. Proteins, 36(2), 205-216.
Czaplewski LG, McKeating J, Craven CJ, Higgins LD, Appay V, Brown A, Dudgeon T, Howard LA, Meyers T, Owen J , Palan SR et al (1999) . J Biol Chem, 274(23), 16077-16084.
Jackson GS, Hill AF, Joseph C, Hosszu L, Power A, Waltho JP, Clarke AR & Collinge J (1999) . Biochim Biophys Acta, 1431(1), 1-13.
Jackson GS, Hosszu LL, Power A, Hill AF, Kenney J, Saibil H, Craven CJ, Waltho JP, Clarke AR & Collinge J (1999) . Science, 283(5409), 1935-1937.
Hurd PJ, Whitmarsh AJ, Baldwin GS, Kelly SM, Waltho JP, Price NC, Connolly BA & Hornby DP (1999) . J Mol Biol, 286(2), 389-401.
Baxter NJ, Hosszu LL, Waltho JP & Williamson MP (1998) . J Mol Biol, 284(5), 1625-1639.
Zerovnik E, Virden R, Jerala R, Turk V & Waltho JP (1998) On the mechanism of human stefin B folding: I. Comparison to homologous stefin A. Influence of pH and trifluoroethanol on the fast and slow folding phases.. Proteins, 32(3), 296-303.
Zerovnik E, Jerala R, Virden R, Kroon Zitko L, Turk V & Waltho JP (1998) . Proteins, 32(3), 304-313.
Estrada S, Nycander M, Hill NJ, Craven CJ, Waltho JP & Björk I (1998) . Biochemistry, 37(20), 7551-7560.
Parker MJ, Dempsey CE, Hosszu LL, Waltho JP & Clarke AR (1998) . Nat Struct Biol, 5(3), 194-198.
Hosszu LL, Craven CJ, Parker MJ, Lorch M, Spencer J, Clarke AR & Waltho JP (1997) . Nat Struct Biol, 4(10), 801-804.
Clarke AR & Waltho JP (1997) . Curr Opin Biotechnol, 8(4), 400-410.
Whitehead B, Craven CJ & Waltho JP (1997) Double and triple resonance NMR methods for protein assignment., 60, 29-52.
Hosszu LL, Craven CJ, Spencer J, Parker MJ, Clarke AR, Kelly M & Waltho JP (1997) . Biochemistry, 36(2), 333-340.
Whitehead B, Craven CJ & Waltho JP (1997) . Methods Mol Biol, 60, 29-52.
Parker MJ, Spencer J, Jackson GS, Burston SG, Hosszu LL, Craven CJ, Waltho JP & Clarke AR (1996) . Biochemistry, 35(49), 15740-15752.
Craven CJ, Whitehead B, Jones SK, Thulin E, Blackburn GM & Waltho JP (1996) . Biochemistry, 35(32), 10287-10299.
Shore P, Whitmarsh AJ, Bhaskaran R, Davis RJ, Waltho JP & Sharrocks AD (1996) . Mol Cell Biol, 16(7), 3338-3349.
Finn BE, Evenäs J, Drakenberg T, Waltho JP, Thulin E & Forsén S (1995) . Nat Struct Biol, 2(9), 777-783.
GROVES P, SEARLE MS, WALTHO JP & WILLIAMS DH (1995) ASYMMETRY IN THE STRUCTURE OF GLYCOPEPTIDE ANTIBIOTIC DIMERS - NMR-STUDIES OF THE RISTOCETIN-A COMPLEX WITH A BACTERIAL-CELL WALL ANALOG. J AM CHEM SOC, 117(30), 7958-7964.
CRAVEN CJ, JONES SK, EVENAS J, DRAKENBERG T, FINN B & WALTHO JP (1995) THE BINDING OF A HYDROPHOBIC DRUG TO THE C-TERMINAL DOMAIN OF CALMODULIN. J CELL BIOCHEM, 70-70.
WALTHO JP, MARTIN JR, CRAVEN CJ, JERALA R, ZEROVNIK E & TURK V (1995) THE REFINED SOLUTION STRUCTURE OF HUMAN STEFIN-A. J CELL BIOCHEM, 40-40.
Shore P, Bisset L, Lakey J, Waltho JP, Virden R & Sharrocks AD (1995) . J Biol Chem, 270(11), 5805-5811.
Martin JR, Craven CJ, Jerala R, Kroon-Zitko L, Zerovnik E, Turk V & Waltho JP (1995) . J Mol Biol, 246(2), 331-343.
CRAVEN CJ & WALTHO JP (1995) THE ACTION OF TIME-DOMAIN CONVOLUTION FILTERS FOR SOLVENT SUPPRESSION. J MAGN RESON SER B, 106(1), 40-46.
Hornby DP, Whitmarsh A, Pinarbasi H, Kelly SM, Price NC, Shore P, Baldwin GS & Waltho J (1994) . FEBS Lett, 355(1), 57-60.
BISSON AP, CARVER FJ, HUNTER CA & WALTHO JP (1994) MOLECULAR ZIPPERS. J AM CHEM SOC, 116(22), 10292-10293.
Martin JR, Jerala R, Kroon-Zitko L, Zerovnik E, Turk V & Waltho JP (1994) . Eur J Biochem, 225(3), 1181-1194.
Ford K, Waltho J & Hornby D (1994) . Biochem J, 298 Pt 3, 727-732.
JENNINGS PA, MERUTKA G, SHIN HC, WALTHO JP, DYSON HJ & WRIGHT PE (1993) FOLDING PATHWAY OF APOMYOGLOBIN. ABSTR PAP AM CHEM S, 206, 107-PHYS.
WALTHO JP & CAVANAGH J (1993) PRACTICAL ASPECTS OF RECORDING MULTIDIMENSIONAL NMR-SPECTRA IN WATER WITH FLAT BASE-LINES. J MAGN RESON SER A, 103(3), 338-348.
Waltho JP, Feher VA, Merutka G, Dyson HJ & Wright PE (1993) . Biochemistry, 32(25), 6337-6347.
Shin HC, Merutka G, Waltho JP, Wright PE & Dyson HJ (1993) . Biochemistry, 32(25), 6348-6355.
Shin HC, Merutka G, Waltho JP, Tennant LL, Dyson HJ & Wright PE (1993) . Biochemistry, 32(25), 6356-6364.
WALTHO JP, LANGDON GM, RICE DW & FITTON JE (1993) STRUCTURE DETERMINATION OF HUMAN ELAFIN. J CELL BIOCHEM, 286-286.
WILLIAMSON MP & WALTHO JP (1992) PEPTIDE STRUCTURE FROM NMR. CHEM SOC REV, 21(4), 227-236.
WILLIAMSON MP, MURRAY NJ & WALTHO JP (1992) IMPROVED EXPERIMENTS FOR THE ASSIGNMENT OF CROWDED PEPTIDE SPECTRA. J MAGN RESON, 100(3), 593-597.
Dyson HJ, Merutka G, Waltho JP, Lerner RA & Wright PE (1992) . J Mol Biol, 226(3), 795-817.
Sweeney PJ, Walker JM, Reid DG, MacLachlan LK & Waltho JP (1991) . Biochem Soc Trans, 19(4), 430S.
WALTHO JP & WILLIAMS DH (1991) THE NATURAL DESIGN OF VANCOMYCIN FAMILY ANTIBIOTICS TO BIND THEIR TARGET PEPTIDES. CIBA F SYMP, 158, 73-91.
Waltho JP & Williams DH (1991) . Ciba Found Symp, 158, 73-86.
WALTHO JP & WILLIAMS DH (1989) . ChemInform, 20(28).
Waltho JP, Feher VA, Lerner RA & Wright PE (1989) . FEBS Lett, 250(2), 400-404.
KAHN SD, BOOTH PM, WALTHO JP & WILLIAMS DH (1989) COMPUTER-ASSISTED STRUCTURE DETERMINATION - STRUCTURE OF THE PEPTIDE MOROIDIN FROM LAPORTEA-MOROIDES. J ORG CHEM, 54(8), 1901-1904.
WALTHO JP & WILLIAMS DH (1989) ASPECTS OF MOLECULAR RECOGNITION - SOLVENT EXCLUSION AND DIMERIZATION OF THE ANTIBIOTIC RISTOCETIN WHEN BOUND TO A MODEL BACTERIAL CELL-WALL PRECURSOR. J AM CHEM SOC, 111(7), 2475-2480.
WILLIAMS DH & WALTHO JP (1989) MOLECULAR-BASIS OF THE ACTIVITY OF ANTIBIOTICS OF THE VANCOMYCIN GROUP. PURE APPL CHEM, 61(3), 585-588.
WALTHO JP, WILLIAMS DH, STONE DJM & SKELTON NJ (1988) . ChemInform, 19(49).
WALTHO JP, WILLIAMS DH, STONE DJM & SKELTON NJ (1988) INTRAMOLECULAR DETERMINANTS OF CONFORMATION AND MOBILITY WITHIN THE ANTIBIOTIC VANCOMYCIN. J AM CHEM SOC, 110(17), 5638-5643.
KANNAN R, HARRIS CM, HARRIS TM, WALTHO JP, SKELTON NJ & WILLIAMS DH (1988) . ChemInform, 19(33).
WALTHO JP, CAVANAGH J & WILLIAMS DH (1988) ASPECTS OF MOLECULAR RECOGNITION - USE OF A TRUNCATED DRIVEN PSEUDO-NOESY EXPERIMENT TO ELUCIDATE THE ENVIRONMENT OF INTERMOLECULAR ELECTROSTATIC INTERACTIONS IN VANCOMYCIN. J CHEM SOC CHEM COMM(11), 707-709.
KANNAN R, HARRIS CM, HARRIS TM, WALTHO JP, SKELTON NJ & WILLIAMS DH (1988) FUNCTION OF THE AMINO SUGAR AND N-TERMINAL AMINO-ACID OF THE ANTIBIOTIC VANCOMYCIN IN ITS COMPLEXATION WITH CELL-WALL PEPTIDES. J AM CHEM SOC, 110(9), 2946-2953.
Waltho JP, Vinter JG, Davis A & Williams DH (1988) . J Comput Aided Mol Des, 2(1), 31-41.
Williams DH & Waltho JP (1988) . Biochem Pharmacol, 37(1), 133-141.
WALTHO JP, WILLIAMS DH, SELVA E & FERRARI P (1987) . ChemInform, 18(52).
WALTHO JP & WILLIAMS DH (1987) STRUCTURE ELUCIDATION OF THE GLYCOPEPTIDE ANTIBIOTIC COMPLEX A40926. J CHEM SOC PERK T 1(9), 2103-2107.
CAVANAGH J, WALTHO JP & KEELER J (1987) SEMISELECTIVE TWO-DIMENSIONAL NMR EXPERIMENTS. J MAGN RESON, 74(2), 386-393.
WALTHO JP, WILLIAMS DH, MAHATO SB, PAL BC & BARNA JCJ (1986) . Chemischer Informationsdienst, 17(51).
WALTHO JP, WILLIAMS DH, MAHATO SB, PAL BC & BARNA JCJ (1986) STRUCTURE ELUCIDATION OF 2 TRITERPENOID TETRASACCHARIDES FROM ANDROSACE-SAXIFRAGIFOLIA. J CHEM SOC PERK T 1(8), 1527-1531.
Waltho J () Peri active site catalysis of proline isomerisation is the molecular basis of allomorphy in β-phosphoglucomutase. Communications Biology.
Falcioni F, Molt RW, Jin Y, Waltho JP, Hay S, Richards NGJ & Blackburn GM () . ACS Catalysis, 6650-6658.
Pandya MJ, Augustyniak W, Cliff MJ, Lindner I, Stinn A, Kahmann J, Temmerman K, Dannatt HRW, Waltho JP & Watson MJ () . Biomolecular NMR Assignments.
Waltho J, Robertson A, Cruz-Navarrete FA, Wood H, Vekaria N, Hounslow A, Bisson C, Cliff M & Baxter N () An enzyme with high catalytic proficiency utilizes distal site substrate binding energy to stabilize the closed state but at the expense of substrate inhibition. ACS Catalysis.
Xanthis I, Souilhol C, Serbanovic-Canic J, Roddie H, Kalli AC, Fragiadaki M, Wong R, Shah DR, Askari JA, Canham L , Akhtar N et al () .
Volk M, Milanesi L, Waltho JP, Hunter CA & Beddard GS () . Physical Chemistry Chemical Physics, 17(2), 762-782.
Bowler MW, Cliff MJ, Waltho JP & Blackburn GM () . ChemInform, 41(34), no-no.
WILLIAMSON MP & WALTHO JP () . ChemInform, 24(12), no-no.
Milanesi L, Trevitt CR, Whitehead B, Hounslow AM, Tomas S, Hosszu LLP, Hunter CA & Waltho JP () .
Milanesi L, Trevitt CR, Whitehead B, Hounslow AM, Tomas S, Hosszu LLP, Hunter CA & Waltho JP () .
Wood HP, Baxter NJ, Trevitt CR, Cruz-Navarrete FA, Hounslow AM & Waltho JP () .
Wood HP, Baxter NJ, Trevitt CR, Cruz-Navarrete FA, Hounslow AM & Waltho JP () .

Chapters

Iorgu AI, Cliff MJ, Waltho JP, Scrutton NS & Hay S (2019) , Methods in Enzymology (pp. 145-166). Elsevier
Waltho JP, Bowler MW, Cliff MJ & Vas M (2013) Architecture of the active site and the importance of charge balance in catalysis, Phosphoglycerate Kinase: A Hinge-Bending Enzyme (pp. 203-210).
Waltho JP, Feher VA & Wright PE (1990) , Current Research in Protein Chemistry (pp. 283-293). Elsevier

Conference proceedings papers

Navarrete FAC, Baxter NJ, Buzoianu A, Wood HP, Hounslow AM & Waltho JP (2021) Substrate discrimination via a proline switch in an allomorphic enzyme. PROTEIN SCIENCE, Vol. 30 (pp 101-101)
Finger LD, Bennet IA, Hounslow A, Exell JC, Baxter NJ, Waltho JP & Grasby JA (2015) The Catalytic Cycle of hFEN1 Requires Protein and DNA Conformational Changes, but Are They Rate-Limiting?. PROTEIN SCIENCE, Vol. 24 (pp 147-147)
Molt R, Jin Y, Pellegrini E, Bowler M, Richards N, Blackburn GM & Waltho J (2015) Extended series of proximal and distal hydrogen bonds underpins RhoA catalyzed GTP hydrolysis via a strain-free transition state. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 250
Waltho J (2013) Kinases, phosphatases, mutases and G-proteins. FEBS JOURNAL, Vol. 280 (pp 93-93)
Leigh KN, Waltho JP, Blackburn GM, Bowler MW & Webster CE (2013) Computational studies of intermediate- and transition-state analogs in b-PGM. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 245
Leigh KN, Waltho JP, Blackburn GM, Bowler MW & Webster CE (2012) Utilization of computed F-19 NMR to identify active site intermediate and transition-state analogs in beta-PGM. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 244
Briggs DC, Ali T, Tongsoongnoen W, Rugg MS, Waltho JP, Levoli E, Mikecz K, Salustri A, Milner CS & Day AJ (2010) Towards the molecular basis of cumulus matrix formation: structure/function studies on TSG-6. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, Vol. 91(6) (pp A31-A31)
Volk M, Milanesi L, Waltho JP, Hunter CA, Dev S, Shaw DJ, Beddard GS & Reid GD (2009) . Biophysical Journal, Vol. 96(3) (pp 322a-323a)
Skerget K, Vilfan A, Waltho JP, Turk D & Zerovnik E (2008) A model for amyloid fibril formation by human stefin B (cystatin B). FEBS JOURNAL, Vol. 275 (pp 199-199)
WRIGHT PE, DYSON HJ, WALTHO JP & LERNER RA (1990) FOLDING OF PEPTIDE-FRAGMENTS OF PROTEINS IN WATER SOLUTION. PROTEIN FOLDING : DECIPHERING THE SECOND HALF OF THE GENETIC CODE (pp 95-+)
WRIGHT PE, DYSON HJ, FEHER VA, TENNANT LL, WALTHO JP, LERNER RA & CASE DA (1990) FOLDING OF PEPTIDE-FRAGMENTS OF PROTEINS IN AQUEOUS-SOLUTION. FRONTIERS OF NMR IN MOLECULAR BIOLOGY, Vol. 109 (pp 1-13)

Preprints

Pellegrini E, Juyoux P, Velsen JV, Baxter NJ, Dannatt HRW, Yi J, Cliff MJ, Waltho JP & Bowler MW (2024) , Cold Spring Harbor Laboratory.
Hosszu LLP, Sangar D, Batchelor M, Risse E, Hounslow AM, Waltho JP, Collinge J & Bieschke J (2022) , Cold Spring Harbor Laboratory.
Hosszu LLP, Sangar D, Batchelor M, Risse E, Hounslow AM, Waltho JP, Collinge J & Bieschke J () .
Wood HP, Baxter NJ, Trevitt CR, Cruz-Navarrete FA, Hounslow AM & Waltho JP () , American Chemical Society (ACS).

Teaching activities

Level 3 modules

MBB302 Physical Methods for Studying Biological Structures
MBB310 Assembly of Supramolecular Structures