Shaw, Jonathan, Dr

Dr Jonathan Shaw

BSc PhD

School of Medicine and Population Health

Reader in Microbiology

j.g.shaw@sheffield.ac.uk
+44 114 215 9553

The Medical School

Full contact details

Dr Jonathan Shaw
School of Medicine and Population Health
The Medical School
Beech Hill Road
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S10 2RX

Profile

For enquiries please contact - SMPH-West-Operational@sheffield.ac.uk

I am a Reader in Microbiology in the School of Medicine and Population Health. After finishing my PhD in 1991, I undertook two short post-doctoral positions in ��«Ӱҵ and Canada before joining the Medical School in 1993 as an Independent Research Fellow.

Research interests

The major direction behind the research in my laboratory is the understanding of bacterial pathogenesis at a molecular and mechanistic level, in relation to colonisation factors, secreted products and physiology.

Current projects

Studies into the pathogenesis and physiology of the pathogenic Neisseria

Neisseria meningitidis is a major cause of bacterial meningitis and Neisseria gonorrhoeae is the causative agent of the sexually transmitted disease gonorrhoea. Although quite a lot is known about these organism's virulence factors, there is very little information available about these organism's carbon metabolism. We are investigating the growth and metabolic characteristics of the pathogenic Neisseria through the use of 13C-NMR and enzyme assays, with the emphasis on growth on lactate. This will enable us to find out what metabolic pathways the organism’s uses in CSF (in vivo) and find out if any unique enzymes are present which can be rationally targeted for antimicrobial therapy.

The role of these pathways in the organism’s pathogenesis are also being considered. We are also investigating the role of the lysine acetylation and investigating the role of the neisserial acetylome on antibiotic resistance and pathogenesis. These projects are in collaboration with Christoph Tang and Rachel Exley at Oxford University.

Use of Aeromonas species as model systems for bacterial colonisation, environmental adaptation and protein glycosylation

Aeromonas spp. are an increasingly important cause of gastro-enteritis and wound infections, with A. caviae being important in the causation of paediatric diarrhoea. However, there is little known about the pathogenicity determinants of this organism.

Some strains of Aeromonas express two distinct flagella systems, a polar flagellum for swimming in liquid environments and many lateral flagella for swarming over surfaces, both are involved in colonisation. Possession of two types of flagella provides a natural reporter system for investigating how bacteria sense surfaces, or for dissecting the bacterial sense of touch.

We are also interested in the genetic cross-talk between the flagellar systems and the type 3 secretion systems (T3SS). In addition to novel effector proteins secreted by the T3SS.

Aeromonas glycosylates its flagellum with the sugar pseudaminic acid, this is essential for flagellar filament assembly and motility. This sugar is also found on the flagellin proteins of Campylobacter jejuni and Helicobacter pylori. We are elucidating the flagellar glycosylation process at the molecular level. We are interested in developing sugar analogues to inhibit the glycosylation process that could possibly be used as a novel form of anti-microbial therapy. These projects are in collaboration with Graham Stafford of the Dental School, Simon Jones in Chemistry and the Universities of Barcelona and Tasmania.

Interactions of Burkholderia with eukaryotic cells

In collaboration with Dr M Thomas (��«Ӱҵ) we are using genetic means such as IVET and mutagenesis to investigate the mechanism of how Burkholderia interacts with host cells via its type VI secretion system.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Parker JL, Lowry RC, Couto NAS, Wright PC, Stafford GP & Shaw JG (2014) . Mol Microbiol, 92(2), 258-272.
Hadi N, Yang Q, Barnett TC, Tabei SMB, Kirov SM & Shaw JG (2012) . Infect Immun, 80(4), 1351-1360.
Parker JL, Day-Williams MJ, Tomas JM, Stafford GP & Shaw JG (2012) . MicrobiologyOpen, 1, 149-160.
Wilhelms M, Molero R, Shaw JG, Tomas JM & Merino S (2011) . J BACTERIOL, 193(19), 5179-5190.
Parker JL & Shaw JG (2011) . J Infect, 62(2), 109-118.
Marteyn B, West NP, Browning DF, Cole JA, Shaw JG, Palm F, Mounier J, Prevost MC, Sansonetti P & Tang CM (2010) . NATURE, 465(7296), 355-U113.
Tabei SMB, Hitchen PG, Day-Williams MJ, Merino S, Vart R, Pang P-C, Horsburgh GJ, Viches S, Wilhelms M, Tomás JM , Dell A et al (2009) . J Bacteriol, 191(8), 2851-2863.
Shalom G, Shaw JG & Thomas MS (2007) . Microbiology, 153(Pt 8), 2689-2699.
Seshadri R, Joseph SW, Chopra AK, Sha J, Shaw J, Graf J, Haft D, Wu M, Ren QH, Rosovitz MJ , Madupu R et al (2006) . J BACTERIOL, 188(23), 8272-8282.
Exley RM, Shaw J, Mowe E, Sun YH, West NP, Williamson M, Botto M, Smith H & Tang CM (2005) . J EXP MED, 201(10), 1637-1645.
Kirov SM, Castrisios M & Shaw JG (2004) Aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces. INFECT IMMUN, 72(4), 1939-1945.
Gavin R, Rabaan AA, Merino S, Tomas JM, Gryllos I & Shaw JG (2002) Lateral flagella of Aeromonas species are essential for epithelial cell adherence and biofilm formation. MOL MICROBIOL, 43(2), 383-397.
Rabaan AA, Gryllos I, Tomás JM & Shaw JG (2001) . Infect Immun, 69(7), 4257-4267.
Leighton MP, Kelly DJ, Williamson MP & Shaw JG (2001) . Microbiology, 147(Pt 6), 1473-1482.

All publications

Journal articles

Lowry RC, Allihaybi L, Parker JL, Couto NAS, Stafford GP & Shaw JG (2022) . MicrobiologyOpen, 11(4).
Green LR, Cole J, Parga EFD & Shaw JG (2022) .
Maldonado‐Arriaga B, Sandoval‐Jiménez S, Rodríguez‐Silverio J, Alcaráz‐Estrada SL, Cortés‐Espinosa T, de Vaca RP, Licona‐Cassani C, Gámez‐Valdez JS, Shaw J, Mondragón‐Terán P , Hernández‐Cortez C et al (2021) . MicrobiologyOpen, 10(2).
Maldonado-Arriaga B, Sandoval-Jiménez S, Rodríguez-Silverio J, Alcaráz- Estrada SL, Cortés-Espinosa T, Pérez-Cabeza de Vaca R, Shaw J, Mondragón-Terán P, Hernández-Cortez C, Suárez-Cuenca JA & Castro-Escarpulli G (2021) . F1000Research, 9.
Flack EKP, Chidwick HS, Guchhait G, Keenan T, Budhadev D, Huang K, Both P, Mas Pons J, Ledru H, Rui S , Stafford GP et al (2020) . ACS Catalysis, 10, 9986-9993.
Gray K, Green LR, Chaudhuri RR & Shaw JG (2019) . Microbiology Resource Announcements, 8(30).
Felix Diaz Parga E & Shaw JG (2019) . Access Microbiology, 1(1A).
Maldonado-Arriaga B, Escobar-Escamilla N, Pérez-Razo JC, Alcaráz-Estrada SL, Flores-Sánchez I, Moreno-García D, Pérez-Cabeza de Vaca R, Mondragón-Terán P, Shaw J, Hernandez-Cortez C , Castro-Escarpulli G et al (2019) . Journal of International Medical Research.
Zhao Y & Shaw J (2016) . Frontiers in Microbiology, 7.
Lowry RC, Parker JL, Kumbhar R, Mesnage S, Shaw JG & Stafford GP (2015) . MicrobiologyOpen, 4(2), 220-234.
Parker JL, Lowry RC, Couto NAS, Wright PC, Stafford GP & Shaw JG (2014) . Mol Microbiol, 92(2), 258-272.
Lowry R, Balboa S, Parker JL & Shaw JG (2014) , 203-256.
Hadi N, Yang Q, Barnett TC, Tabei SMB, Kirov SM & Shaw JG (2012) . Infect Immun, 80(4), 1351-1360.
Parker JL, Day-Williams MJ, Tomas JM, Stafford GP & Shaw JG (2012) . MicrobiologyOpen, 1, 149-160.
Wilhelms M, Molero R, Shaw JG, Tomas JM & Merino S (2011) . J BACTERIOL, 193(19), 5179-5190.
Parker JL & Shaw JG (2011) . J Infect, 62(2), 109-118.
Marteyn B, West NP, Browning DF, Cole JA, Shaw JG, Palm F, Mounier J, Prevost MC, Sansonetti P & Tang CM (2010) . NATURE, 465(7296), 355-U113.
Wilhelms M, Vilches S, Molero R, Shaw JG, Tomas JM & Merino S (2009) . J BACTERIOL, 191(7), 2206-2217.
Tabei SMB, Hitchen PG, Day-Williams MJ, Merino S, Vart R, Pang P-C, Horsburgh GJ, Viches S, Wilhelms M, Tomás JM , Dell A et al (2009) . J Bacteriol, 191(8), 2851-2863.
Shalom G, Shaw JG & Thomas MS (2007) . Microbiology, 153(Pt 8), 2689-2699.
Canals R, Vilches S, Wilhelms M, Shaw JG, Merino S & Tomas JM (2007) . MICROBIOL-SGM, 153, 1165-1175.
Exley RM, Wu H, Shaw J, Schneider MC, Smith H, Jerse AE & Tang CM (2007) . INFECT IMMUN, 75(3), 1318-1324.
Seshadri R, Joseph SW, Chopra AK, Sha J, Shaw J, Graf J, Haft D, Wu M, Ren QH, Rosovitz MJ , Madupu R et al (2006) . J BACTERIOL, 188(23), 8272-8282.
Merino S, Shaw JG & Tomas JM (2006) . FEMS MICROBIOL LETT, 263(2), 127-135.
Canals R, Ramirez S, Vilches S, Horsburgh G, Shaw JG, Tomas JM & Merino S (2006) . J BACTERIOL, 188(8), 3166-3166.
Canals R, Altarriba M, Vilches S, Horsburgh G, Shaw JG, Tomas JM & Merino S (2006) . J BACTERIOL, 188(8), 3167-3167.
Canals R, Altarriba M, Vilches S, Horsburgh G, Shaw JG, Tomas JM & Merino S (2006) . J BACTERIOL, 188(3), 852-862.
Canals R, Ramirez S, Vilches S, Horsburgh G, Shaw JG, Tomas JA & Merino S (2006) . J BACTERIOL, 188(2), 542-555.
Rock JD, Mahnane MR, Anjum MF, Shaw JG, Read RC & Moir JWB (2005) . MOL MICROBIOL, 58(3), 800-809.
Exley RM, Goodwin L, Mowe E, Shaw J, Smith H, Read RC & Tang CM (2005) . INFECT IMMUN, 73(9), 5762-5766.
Exley RM, Shaw J, Mowe E, Sun YH, West NP, Williamson M, Botto M, Smith H & Tang CM (2005) . J EXP MED, 201(10), 1637-1645.
Kirov SM, Castrisios M & Shaw JG (2004) Aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces. INFECT IMMUN, 72(4), 1939-1945.
Subsin B, Thomas MS, Katzenmeier G, Shaw JG, Tungpradabkul S & Kunakorn M (2003) . J BACTERIOL, 185(23), 7008-7014.
Gavin R, Merino S, Altarriba M, Canals R, Shaw JG & Tomas JM (2003) . FEMS MICROBIOL LETT, 224(1), 77-83.
Altarriba M, Merino S, Gavin R, Canals R, Rabaan A, Shaw JG & Tomas JM (2003) . MICROB PATHOGENESIS, 34(5), 249-259.
Merino S, Gavin R, Vilches S, Shaw JG & Tomas JM (2003) . APPL ENVIRON MICROB, 69(1), 663-667.
Kirov SM, Tassell BC, Semmler ABT, O'Donovan LA, Rabaan AA & Shaw JG (2002) Lateral flagella and swarming motility in Aeromonas species. J BACTERIOL, 184(2), 547-555.
Gavin R, Rabaan AA, Merino S, Tomas JM, Gryllos I & Shaw JG (2002) Lateral flagella of Aeromonas species are essential for epithelial cell adherence and biofilm formation. MOL MICROBIOL, 43(2), 383-397.
Rabaan AA, Gryllos I, Tomás JM & Shaw JG (2001) . Infect Immun, 69(7), 4257-4267.
Leighton MP, Kelly DJ, Williamson MP & Shaw JG (2001) . Microbiology, 147(Pt 6), 1473-1482.
Lowe CA, Asghar AH, Shalom G, Shaw JG & Thomas MS (2001) . Microbiology, 147(Pt 5), 1303-1314.
Gryllos I, Shaw JG, Gavín R, Merino S & Tomás JM (2001) . Infect Immun, 69(1), 65-74.
Shalom G, Shaw JG & Thomas MS (2000) . Biotechniques, 29(5), 954-958.
Thornley JP, Shaw JG, Gryllos IA & Eley A (1997) Virulence properties of clinically significant Aeromonas species: Evidence for pathogenicity. REV MED MICROBIOL, 8(2), 61-72.
Thornley JP, Shaw JG, Gryllos IA & Eley A (1996) . J Med Microbiol, 45(6), 445-451.
Eley A, Shaw JG, Thornley JP & Geary I (1996) Isolation and initial identification of adherence-related extracellular appendages in Aeromonas caviae. MED MICROBIOL LETT, 5(1), 40-45.
THORNLEY JP, SHAW JG, GRYLLOS IA & ELEY A (1995) CELL-LINE ADHESION AND HEMAGGLUTINATION OF AEROMONAS-CAVIAE CLINICAL ISOLATES. MED MICROBIOL LETT, 4(6), 316-323.
SHAW JG, THORNLEY JP, PALMER I & GEARY I (1995) INVASION OF TISSUE-CULTURE CELLS BY AEROMONAS-CAVIAE. MED MICROBIOL LETT, 4(6), 324-331.
Thornley JP, Eley A, Shaw JG & Neves MS (1994) . Journal of Clinical Microbiology, 32(10), 2631-2632.
FORD I, DOUGLAS CWI, SHAW J, HAMPTON KK & PRESTON FE (1993) THE INTERACTION BETWEEN BACTERIAL-CELLS AND A PLASMA COMPONENT IS AN ESSENTIAL STEP IN THE PLATELET-AGGREGATION RESPONSE TO STREPTOCOCCUS-SANGUIS. THROMB HAEMOSTASIS, 69(6), 909-909.
Hamblin MJ, Shaw JG & Kelly DJ (1993) . Mol Gen Genet, 237(1-2), 215-224.
Walmsley AR, Shaw JG & Kelly DJ (1992) . Biochemistry, 31(45), 11175-11181.
Walmsley AR, Shaw JG & Kelly DJ (1992) The mechanism of ligand binding to the periplasmic C4-dicarboxylate binding protein (DctP) from Rhodobacter capsulatus.. J Biol Chem, 267(12), 8064-8072.
Shaw JG, Hamblin MJ & Kelly DJ (1991) . Mol Microbiol, 5(12), 3055-3062.
SHAW JG & KELLY DJ (1991) BINDING-PROTEIN DEPENDENT TRANSPORT OF C4-DICARBOXYLATES IN RHODOBACTER-CAPSULATUS. ARCH MICROBIOL, 155(5), 466-472.
Hamblin MJ, Shaw JG, Curson JP & Kelly DJ (1990) Mutagenesis, cloning and complementation analysis of C4-dicarboxylate transport genes from Rhodobacter capsulatus.. Mol Microbiol, 4(9), 1567-1574.
Vasey B, Nagendran M, Campbell B, Clifton DA, Collins GS, Denaxas S, Denniston AK, Faes L, Geerts B, Ibrahim M , Liu X et al () . Nature Medicine.
Shaw JG, Thornley J & Eley A () Adherence and invasion of Aeromonas caviae to monolayer cells.. Advances in Experimental Medicine and Biology, 412, 217-219.

Chapters

Shaw JG & Parker J (2015) Aeromonad antigenic structures In Graf J (Ed.), Aeromonas (pp. 117-137). Norfolk, UK: Caister Academic Press.
Kelly DJ, Hamblin MJ & Shaw JG (1990) Physiology and genetics of C4-dicarboxylate transport in Rhodobacter capsulatus, Molecular Biology of Membrane Bound complexes in Phototrophic bacteria (pp. 453-462). Plenum Press

Conference proceedings papers

Bull EH, Bardhan KD & Shaw JG (2007) Lareal flagella regulation of the enteropathogen Aeromonas hydrophila. Gastroenterology, Vol. 132(4) (pp A712-A713)
Bull EH, Bardhan KD & Shaw JG (2007) Lateral flagella regulation of the enteropathogen Aeromonas hydrophila. GASTROENTEROLOGY, Vol. 132(4) (pp A712-A713)
Horsburgh GJ, Clark S, Kirov SM & Shaw J (2003) Regulation of the lateral flagella colonisation systemof Aeromonas caviae. FEMS Congress of European Microbiologists, Vol. 1 (pp 450-450)
Shaw JG (1997) Cromps fimbriae and flagella: the quest for the adhesins of Aeromonas. Journal of Medical Microbiology, Vol. 46 (pp 1047-1048)
Thornley JP, Naz SM, Eley A & Faulkner M (1994) Investigations into adherence mechanisms of Aeromonas caviae. Journal of Medical Microbiology, Vol. 41 (pp 277-277)
Thornley JP, Naz SM, Eley A & Shaw J (1994) Adherence and haemagglutination of Aeromonas caviae. Journal of Medical Microbiology, Vol. 41 (pp 277-277)
Ford I, Douglas CWI, Shaw J, Hampton K & Preston FE (1993) The interaction between bacterialcells and a plasma component is an essentila step in the platelet aggregation response to Streptococcus sanguis. Thrombosis and haemostasis, Vol. 69 (pp 909-909)

Preprints

Wolverson PA, Fernandes Parreira I, Collins MO, Shaw JG & Green LR (2024) , Cold Spring Harbor Laboratory.
Maldonado-Arriaga B, Sandoval-Jiménez S, Rodríguez-Silverio J, Alcaráz- Estrada SL, Cortés-Espinosa T, Pérez-Cabeza de Vaca R, Shaw J, Mondragón-Terán P, Hernández-Cortez C, Suárez-Cuenca JA & Castro-Escarpulli G () .
Maldonado-Arriaga B, Sandoval-Jimenez S, Rodríguez-Silverio JR-S, Alcaráz-Estrada SL, Cortés-Espinosa T, Vaca RP-CD, Licona-Cassani C, Gámez-Valdez JS, Shaw J, Mondragón-Terán P , Hernández-Cortez C et al () , Research Square Platform LLC.

Teaching interests: I am heavily involved in the M.Sc. in Molecular Medicine, M.Sc. Genomic Medicine, and I also teach on the Medicine, Dental, M.Sc Antimicrobial resistance and Orthoptics courses.

Professional activities and memberships

I was previously on the Prokaryotic Division of the Microbiology Society (UK) and on the editorial board of the journal Microbiology for eight years.

I am currently an associate editor for MicrobiologyOpen, Frontiers in Microbiology, Frontiers in Cellular and Infection Microbiology and the journal Virulence.