Dr Neil Chapman
B.Sc.(Hons.), Ph.D., P.G.Cert.H.E., S.F.H.E.A.
School of Medicine and Population Health
Senior University Teacher Reproductive Medicine
+44 114 215 9671
Full contact details
School of Medicine and Population Health
JW4/54, Level 4
Jessop Wing
Tree Root Walk
ºù«Ӱҵ
S10 2SF
- Profile
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For enquiries please contact - SMPH-West-Operational@sheffield.ac.uk
I joined the University of ºù«Ӱҵ as a Group Leader and Non-Clinical Lecturer in Reproductive Medicine in July 2005. Previous research positions included:
- July 2005-December 2017
Lecturer in Reproductive Medicine, Academic Unit of Reproductive and Developmental Medicine, University of ºù«Ӱҵ - October 2003 - June 2005
Post-Doctoral Research Associate, School of Surgical and Reproductive Sciences, University of Newcastle-upon-Tyne. Research: Transcriptional regulation in the myometrium and cervix by the Nuclear Factor kappaB (NF-kappaB) and novel membrane-bound steroid receptors - June 1997 - September 2003
Post-Doctoral Research Associate, School of Life Sciences, Division of Gene Expression and Regulation, Wellcome Trust Biocentre, University of Dundee. Research: Transcriptional regulation by NF-kappaB; determining how NF-kappaB regulates mammalian gene expression in conjunction with other proteins (Egr-1, WT-1, CBP/p300 and c-Myc) with an emphasis on tumourigenesis - September 1994 - May 1997
May 1997 PhD student, University of ºù«Ӱҵ. Research: Expression and characterisation of the ZP3 protein; to develop purification strategies to isolate recombinant copies of ZP3 and then characterise their effects on human spermatozoa
- July 2005-December 2017
- Research interests
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Premature Birth
Globally, being born too early affects roughly 15 million pregnancies per year. Of these, about 1 million babies will die because they were born too early and suffered complications of that. The majority of these deaths are in those babies that are born extremely prematurely: before 28-30 weeks gestation. To put this number into context, here in ºù«Ӱҵ the Jessop Maternity Wing manages in the region of 7,000 deliveries per year. To reach the annual global death toll attributable to premature birth there would need to be no live births in the Jessop for approximately the next 140 years.
See how staff and local people help to raise the profile of premature birth research at the .
We also know that those babies that do survive prematurity also have an increased risk of major long-term health problems. It is principally because of these observations that these babies have a disproportionate effect on health-care budgets worldwide; a recent U.K. estimate of the total cost of preterm birth to the public sector over the first 18 year of life was £2.95 billion. Therefore, being born too early is a very much a problem for life. For those readers wanting further details of this, please visit the .
So why does premature birth happen? The simple answer is that despite intensive research efforts, together with a major shortage of research funding into this problem, we still don’t know enough about the fundamental biological principles that control how the womb works during pregnancy and labour. As such, it is of little surprise that doctors have few truly effective medicines to stop labour when it has started too early. Those drugs that are available are relatively ineffective and can be associated with unwanted side-effects for both the unborn baby and mother.
My research work uses muscle cells from the womb (called myocytes). We get these samples by asking women to donate a piece of their womb lining when they have an elective Caesarean section. From this piece of womb muscle we can then grow the individual muscle cells and study how they work.
Within the womb muscle cells are structures called the nuclei. It is within each nucleus that the blueprint of the womb muscle cell (the DNA or genome) is stored and used to control when the womb starts to contract. The genome or DNA can be thought of as a library of instruction books. Each book represents an individual gene, with each gene being used to make a specific building block - a protein - in the cell. Importantly, however, these books are not written with words: only four letters are used – A, T, C, and G – and different arrangements of these letters are what gives rise to all the different proteins in your body as well telling cells what proteins to make and when to make them.
In humans, the biology by which the womb changes from a relaxed state which cannot contract regularly, to an organ which undergoes the regular contractions seen in labour is not known. At present, our current knowledge about the human birth process suggests that normal human labour is a highly-regulated inflammatory process similar to that which occurs when the body is injured or ill.
My work has shown that the inflammation seen in the womb at term modifies how the blueprint of the womb muscle cells (the DNA or genome) works to regulate when the womb contracts with inflammation seemingly able to promote labour (). Consequently, my research focuses on learning how the womb reads its genome book when such inflammatory chemicals are present. I am also interested in finding out which parts of the book (i.e. which genes) the womb cells use before labour starts, when there is very little inflammation around, because understanding this change may allow us to understand how the womb muscle cells start contracting too early in some women who then go into labour prematurely.
- Publications
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Show: Featured publications All publications
Featured publications
Books
- SBAs for the Part 1 MRCOG. Royal College of Obstetricians and Gynaecologists, 27 Sussex Place, Reagent's Park, London: Royal College of Obstetricians and Gynaecologists Press.
Journal articles
- . Wilderness and Environmental Medicine.
- . Extreme Physiology and Medicine, 6(1).
- Immediate Care Skills for Your Elective: Two simple frameworks to help you wherever you are. Student BMJ, 24.
- . Molecular Human Reproduction, 21(11), 865-883.
- . Biochemical Journal, 366(2), 459-469.
- . Frontiers in Physiology, 5.
- Regulation of GTP-binding Protein (Galpha s) Expression in
Human Myometrial Cells
A ROLE FOR TUMOR NECROSIS FACTOR IN MODULATING G s PROMOTER ACETYLATION
BY TRANSCRIPTIONAL COMPLEXES. Journal of Biological Chemistry, 288(9), 6704-6716.
Chapters
- NF-kappaB Function in Inflammation, Cellular Stress and Disease In Storey JM & Storey KB (Ed.), Sensing, Signaling and Cell Adaptation: 3 (pp. 61-73). Elsevier Science
All publications
Books
- SBAs for the Part 1 MRCOG. Royal College of Obstetricians and Gynaecologists, 27 Sussex Place, Reagent's Park, London: Royal College of Obstetricians and Gynaecologists Press.
Journal articles
- . BMC Medical Education, 23(1).
- . Wilderness and Environmental Medicine.
- . Extreme Physiology and Medicine, 6(1).
- . The BMJ, 353.
- Immediate Care Skills for Your Elective: Two simple frameworks to help you wherever you are. Student BMJ, 24.
- . Molecular Human Reproduction, 21(11), 865-883.
- Human Trophoblast Cells Modulate Endometrial Cells Immune Response to Flagellin In Vitro. PLoS One, 8(1), e39441.
- . Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health, 1, S45-S45.
- . Histol Histopathol, 25(7), 945-956.
- . Fertility and Sterility, 94, 833-840.
- The Expression of Inducible Nitric Oxide Synthetase (iNOS) and MUC1 in Human Fallopian Tube during the Menstrual Cycle and in Ectopic Pregnancy. REPRODUCTIVE SCIENCES, 16(3), 187A-187A.
- . Journal of Clinical Endocrinology and Metabolism, 93, 293-299.
- Semi-quantitative analysis of TLR2 and TLR4 expression in the non-pregnant and pregnant cervix and the potential role of oestrogen. BJOG-INT J OBSTET GY, 115(1), 123-123.
- . European Urology Supplements, 6(2), 144-144.
- . Mol Hum Reprod, 12(1), 19-24.
- . Endocrinology, 146(11), 4994-5002.
- Expression and interactions of the transcriptional co-regulators CBP/p300 in the human myometrium during pregnancy.. J. Soc. Gynaecol. Invest., 12, 92-97.
- . Journal of Clinical Endocrinology and Metabolism, 89(11), 5683-5693.
- , 61-73.
- . Biochemical Journal, 366(2), 459-469.
- . Biochemical Society Transactions, 29(6), 688-688.
- UV stimulation induces nuclear factor kappaB (NF-kappaB) DNA-binding activity but not transcriptional activation.. Biochemical Society Transactions, 29, 688-691.
- . Journal of Biological Chemistry, 275, 4719-4725.
- . Biochemical Society Transactions, 27(3), A99-A99.
- The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro. BIOCHEM J, 330, 839-845.
- . Mol Hum Reprod, 3(8), 646-650.
- The polypeptide backbone of recombinant human ZP3 induces acrosomal exocytosis in human spermatozoa. HUM REPROD, 12(5), S13-S13.
- . Mol Hum Reprod, 2(10), 767-774.
- . Frontiers in Physiology, 5.
- Regulation of GTP-binding Protein (Galpha s) Expression in
Human Myometrial Cells
A ROLE FOR TUMOR NECROSIS FACTOR IN MODULATING G s PROMOTER ACETYLATION
BY TRANSCRIPTIONAL COMPLEXES. Journal of Biological Chemistry, 288(9), 6704-6716.
- . PLoS ONE, 8(1), e39441-e39441.
Chapters
- , Part 1 MRCOG Synoptic Revision Guide (pp. 272-284). Cambridge University Press
- NF-kappaB Function in Inflammation, Cellular Stress and Disease In Storey JM & Storey KB (Ed.), Sensing, Signaling and Cell Adaptation: 3 (pp. 61-73). Elsevier Science
Conference proceedings papers
- Regulation of CyclinD2 by Smad3 and Foxl2 during early follicle development. Society for Reproduction and Fertility Annual Meeting. Oxford, UK, 20 July 2015 - 22 July 2015.
- Changes in Expression of PKA-and Acetylation-Related Signalling Molecules with Guinea Pig Pregnancy. REPRODUCTIVE SCIENCES, Vol. 19(S3) (pp 196A-196A)
- Identifying Regulatory Gene Networks in the Myometrium Governed by NF-kappa B. REPRODUCTIVE SCIENCES, Vol. 16(3) (pp 113A-113A)
- Semi-quantitative analysis of TLR2 and TLR4 expression in the non-pregnant and pregnant cervix and the potential role of oestrogen.. REPRODUCTIVE SCIENCES, Vol. 15(2) (pp 188A-188A)
- Temporal expression of the membrane-bound estrogen receptor, GPR30, in lower and upper segment human myometrium.. JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION, Vol. 13(2) (pp 240A-240A)
- Regulation of GaS expression by TNF alpha and the nuclear factor kappaB RelA and p50 subunits.. JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION, Vol. 12(2) (pp 251A-251A)
- Differentail expression of the membrane-bound progesterone receptor alpha isoform in human cervix and lower segment myometrium.. JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION, Vol. 12(2) (pp 288A-289A)
- Expression and interactions of the transcriptional co-regulators CBP/p300 in the human myometrium during pregnancy.. JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION, Vol. 11(2) (pp 76A-76A)
- The production and purification of recombinant human ZP3 from E-6coli. HUMAN SPERM ACROSOME REACTION, Vol. 236 (pp 404-405)
- The signal transduction pathway of the acrosome reaction in human spermatozoa in response to purified recombinant human ZP3. HUMAN SPERM ACROSOME REACTION, Vol. 236 (pp 426-427)
- Foxl2 and Smad transcription factors during early follicle development: localisation and regulation of target genes. Society for Reproduction and Fertility Annual Meeting. Edinburgh, UK, 1 September 2014 - 2 September 2015.
- SBAs for the Part 1 MRCOG. Royal College of Obstetricians and Gynaecologists, 27 Sussex Place, Reagent's Park, London: Royal College of Obstetricians and Gynaecologists Press.
- Professional activities and memberships
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- Academic Lead - Phase 3A SSC Programme (Medicine)
- Academic Lead - Phase 3B Electives (Medicine)
- Academic Lead – Incoming International Electives Programme (Medicine)
- Admissions Tutor - Incoming Medical Electives Programme (/medicine/electives)
- Admissions Tutor - M.Sc. Reproductive and Developmental Medicine (/humanmetabolism/units/rdm/msc)
- Deputy Module Leader – Physiology Module of the M.Sc. Human Nutrition (/postgraduate/taught/courses/medicine/biomedical/human-nutrition-mmedsci-diploma)
- Invited speaker - Part 1 MRCOG Revision Course at the Royal College of Obstetricians and Gynaecologists, London.
- Member - The MRC-Arthritis Research UK Centre for Integrated Research into Musculoskeletal Ageing (CIMA) (www.sheffield.ac.uk/humanmetabolism/mresmusculoskeletalageing/cima)